Spectrophotometric Determination of Amino Acids
نویسندگان
چکیده
منابع مشابه
The Microbiological Determination of Amino Acids
After the development of methods for the determination of valine, leucine, and isoleucine (1) with LactobacilEus arabinosus 17-5 as the test organism, it was assumed that other amino acids essential for the growth of this organism could be determined by about the same procedure. However, it was soon discovered that glutamic acid is unique in its relationship to the growth of Lactobacillus arabi...
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Transamination of branched-chain amino acids to (alpha-oxoglutarate, catalyzed by leucine aminotransferase coupled to the glutamate dehydrogenase reaction, is used in an enzymic assay for determination of branched-chain amino acids in serum and tissue homogenates. The coefficients of variation of the method within-day and day-to-day are 2.4 and 6.5%, respectively. Analytical recovery of physiol...
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For a dietary amino acid to be retained in tissue protein in an animal, the amino acid needs to be ingested by the animal and absorbed from the intestinal tract. While the absorption of amino acids is a concept that can easily be defined, it is difficult to measure. Instead, the digestibility of amino acids is measured. Digestibility is defined as the difference between the amount of a certain ...
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1. An acid ninhydrin reagent was found to react specifically in forming a pink product (E(max.) 560mmu) with cysteine. 2. The method was highly sensitive for the determination of cysteine (in 28.0x10(3)). Homocysteine, glutathione, proline, ornithine and other naturally occurring amino acids tested did not give a similar reaction. 3. The reaction product was stable for at least 3-4hr. at room t...
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The utility of Patton and Reeder’s indicator, HHSNNA, for the trace spectrophotometric and visual colorimetric determination of Cu 2+ is explored. A wine red complex of composition 1:1 is found to be formed between Cu 2+ and HHSNNA. The complex has λmax at 530 nm with ε = 25,558 L mole -1 cm -1 and is stable for more than 2 hrs. Beer’s law is obeyed between 0 to 1.5 ppm. The developed procedure...
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ژورنال
عنوان ژورنال: Journal of the agricultural chemical society of Japan
سال: 1954
ISSN: 0002-1407,1883-6844
DOI: 10.1271/nogeikagaku1924.28.775